![]() The use of selective detergent extraction eliminates the hassle of phase separation based on hydrophobicity, allowing better reproducibility and higher throughput. The Mem-PER Plus Membrane Protein Extraction Kit is for the enrichment of integral membrane proteins and membrane associated proteins from cultured mammalian cells or tissue using a mild detergent-based selective extraction protocol. Traditional methods for isolation of membrane protein are tedious and time-consuming, and they require gradient separation and expensive ultracentrifugation equipment. Kit contains a cell wash solution, permeabilization buffer, and solubilization buffer. ![]() The protocol is simpler, and the fractions are directly compatible with many downstream applications such as SDS-PAGE, Western Blotting, BCA, immunoprecipitation, and amine-reactive protein labeling techniques. The Mem-PER Plus Kit has many advantages compared to our original Mem-PER Kit. The kit makes possible the extraction and selective enrichment of integral and attached membrane proteins from cultured mammalian cells, as well as from hard and soft mammalian tissues. Integral membrane proteins having one or two membrane-spanning domains are effectively solubilized and isolated from cytosolic proteins. The Mem-PER Plus Kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
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